CleaScrip T7 RNA Polymerase is an enzyme used for synthesizing RNA from a DNA template in vitro.
The "Low dsRNA" formulation is specifically designed to minimize double-stranded RNA (dsRNA) contaminants, which can be problematic in certain applications.

In Vitro Transcription (IVT) for mRNA Synthesis

Generate high yields of mRNA for research and vaccines.
Efficient transcription with high fidelity, yielding mRNA with minimal dsRNA contamination.

Produces pure mRNA, such as mRNA vaccines, RNA Probes Synthesis

Produce RNA probes for hybridization-based assays.

High-quality single stranded RNA probes with clear, specific hybridization signals and low background noise.

The low dsRNA feature reduces nonspecific binding, enhancing assay sensitivity and accuracy.
RNA Interference (RNAi) Studies with small interfering RNA (siRNA) for gene silencing
Effective transcription of siRNA with minimal off-target effects due to low dsRNA levels.
Consist and reliablein gene knockdown

Cleascript offer superior High RNA yields, essential for downstream applications.

Low dsRNA Contamination

Significant reduction in dsRNA levels compared to other commercially available T7 RNA polymerases.

High purity verified by gel electrophoresis and spectrophotometric analysis.

Superior dsRNA Control and improvement in reducing dsRNA contaminants compared to standard T7 RNA polymerases.

Ensure DNA template purity to maximize enzyme efficiency.

CleaScrip T7 RNA Polymerase Low dsRNA, 250 U/μL, is highly regarded by mRNA Labs for its efficiency, reliability, and superior quality in RNA synthesis applications.

Its ability to minimize dsRNA contamination makes it an invaluable tool in various RNA-based research and therapeutic applications.

Lieven Gevaert, Bio-ir RUG 1996

This product is a bacteriophage T7 RNA polymerase derived from recombinant protein expression in Escherichia coli. It catalyses the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.

The DNA with double-stranded linear blunt ends or 5'protruding ends can be used as templates for T7 RNA polymerase, so linearized plasmids and PCR products can be used as templates for in vitro synthesis of RNA. Note: G* is the first base of the RNA transcript. This product is produced in accordance with GMP regulations and provided in liquid form.

Feature Synthesize long transcripts and short transcripts, RNA can be produced 100-200 μg with 1 μg of DNA template Higher yield and easy to operate Lower endotoxins Tested for the absence of endonucleases, exonucleases, RNases Application Radiolabelled RNA probe preparation RNA generation for studies of RNA structure, processing and catalysis Expression control via anti-sense RNA mRNA, sgRNA synthesis

Note: Cleascript is a wrong denomination of Cleascrip also found in NCBI publications.