• SeekSpace® offers a cost-effective, QC-focused solution that balances spatial resolution and transcriptomic integration, making it ideal for broad applications in spatial biology.
• MERFISH excels in multiplexing and spatial accuracy but demands specialized infrastructure.
• 10x Genomics Visium provides a user-friendly platform with extensive support, though with higher costs.
• Emerging techniques like Slide-seq and GeoMx DSP cater to niche applications, emphasizing either resolution or targeted profiling.

For researchers seeking a comprehensive balance of cost, QC, and precision, SeekSpace® is an excellent choice, especially for studies requiring high nuclear density and stringent RNA quality control. For multiplexing-heavy projects or highly localized RNA visualization, MERFISH and Slide-seq are superior alternatives.